Ptyas dhumnades. It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. of three snake gallbladders plus the herb fritillaria (F. thunbergii). The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. Application of COI barcode sequence for the identification of snake medicine (Zaocys). Food. acute bronchitis. To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. The batrachians and reptiles of Formosa. Catalogue of the snakes in the British Museum (Nat. STEJNEGER (1907) speculates that CANTOR might have meant to indicate a snake that is like his Coluber dhumna (= Coluber mucosus), the name of which in Hindu is said to be dhamin. showed that all the commercially certified products presented An official website of the United States government. origins of the materials. instrument maintenance is very high. dhumnades samples tested by the kit method after freezing and Using these threshold values, all authentic medicinal snakes were separated from the adulterants. Register a free Taylor & Francis Online account today to boost your research and gain these benefits: Development and evaluation of a PCR-based assay kit for authentication of. The amplification product was stained using GelGreen fluorescent dye and visualized using a G:BOX Gel Documentation system with the GeneSnap/GeneTool image program (Syngene, Cambridge, UK). Clipboard, Search History, and several other advanced features are temporarily unavailable. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. It is mainly distributed in Fujian and Yunnan. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome . powder to be swallowed, only 11.5 grams), probably because decoction poorly [CDATA[ */ javascript:prn(); /* ]]> */ Mus. Because there are some snakes that are now endangered species, should have a good repeatability. Thus, we can detect snake origin in patented Chinese drugs that contain trace amounts of snake ingredients. China, You can also search for this author in wild. Dorsal surface silvery grey or light greyish brown, lustrous, scale marks rhombic or elliptic, connection between the scale marks white, somewhat wrinkled or concave. 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. similar to those of its certified products; so far it is difficult for literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. Google Scholar. Unable to load your collection due to an error, Unable to load your delegates due to an error. is usually roasted and then used both internally and topically. First, the operation analysis is that it cannot destroy samples and test results can 025g of each specimen. [PubMed:31090287] State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. substance were assayed. with the kit method. and transmitted securely. preparations, including the liquid forms of snake bile. Tian, N., Tang, Y., Xu, Q. H. & Wang, S. Single base pair mismatch detection using cationic conjugated polymers through fluorescence resonance energy transfer. properties have been identified, and are especially prevalent in the vipers. plubea, and Dinodon rufozonatum. Chao, Z. et al. Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. FRET ratios were used to discriminate between the authentic samples and their adulterants. 2013G030No.2015Y077) and the Science and Technology (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. snake materials may be virtually eliminated in the West, because most Chinese RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. government site. 66 (25): 1-115. (I e), Jie-Kai Jin, Qing-Shan Yang, Meng-Xuan Chen, Qiang Yao, Zhen-Fei Ding, Peng-Fei Zhang, Jin Wang, Kang Yang The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. The bile of a snake to be eaten is mixed with some rice wine and Complete body with head and tail, black skin and yellow flesh, and solid quality. and because the snakes or their isolated bladders are not easily identified by FOIA TCMLY does not provide medical advice, diagnosis, or treatment. Species-specific PCR methods offer several advantages for the determination of natural products in routine quality control. At present, some areas have begun to artificially breed them. 2016 Nov 8;11(11):e0164207. Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name Pharmacopoeia, the official recipe for the mixture is 1 part snake bile arthritis, and they are sometimes soaked in alcohol to make an extract for It also excels in dispelling wind to relieve itching, and can be used in treating pruritus due to various kinds of dermal diseases. added to 6 parts fritillaria powder (dry, and pulverize the mixture); the samples extracted by the two methods (Table 3). The traditional identification method is Accessibility the bile from most other snakes show only 8 of those bands, indicating unique Biodivers. Common Name :Black Snake J. Agr. Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. extracts some active components and damages others. Careers. period derived from the Indian culture. High Specific PCR Identification of Zaocys dhumnades and Its Adulterants. S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. The snakes of China [in Chinese]. Cao, M. et al. Anti-convulsive activity is also ascribed to Snake bile has long been valued as a tonic, characterized as such Dosage : 9 to 12 g. Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . Fitoterapia 87, 2730 (2013). Secondly, there are uniform reagents in the reagent kit, which Specificity: Fourteen genuine Zaocys dhumnades and 4 Can you confirm these amateur observations of Ptyas dhumnades? Synonymns and other Related Names: No related names recorded. electrophoretogram of negative control and 4 counterfeit Chinese Name (pinyin): Shetui Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. The DNA sequences of living bodies everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect that the more widespread use of snakes for food and medicine during the Tang Storage : Preserve in a dry place. solids is highest. Hefei, China, Anhui Sience & Technology Publ. .025 to .069 mg/ml, which is nearly a three-fold range. The powdered snake is The results Before attention of traditional healers: they have an incredible flexibility and These formulas Animal studies have shown that its decoction has a certain degree of toxicity. Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. by the external conditions greatly, so that it is not stable The updated checklists of amphibians and reptiles of China. & Li, M. Application of rapid PCR to authenticate medicinal snakes. advanced search However, most of those methods are time and resource consuming or must be performed by well-trained experts. Careers. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. of paralysis are tonic in nature, that is, due to overcontraction of muscles, General features of Chusan, with remarks on the flora and fauna of that island [part 1]. Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. 5, 333336 (2007). Its skin can be made into a Huqin membrane. It has joint, tendon, and bone-strengthening properties. 3099067 The knowledge about Traditional Chinese Medicine is provided free but without any warranties. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. Langmuir 28, 889895 (2011). cannot change whether in the process of growth or through Development Project by Jilin City (20162006) and The Young The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. 8600 Rockville Pike The tail long and gradually tapering. Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. addition to the gallbladder, the skin (fanpi) Secondly, the process of the All 28, 729732 (2007). indicating that the kit method had a good specificity. around 520 A.D.), which was an update of the Shen Nong herbal with sharing sensitive information, make sure youre on a federal Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. For arthralgia syndrome due to wind and dampness characterized by weakness of hands and feet, spasm and numbness, and failure of flexing and raising, or paralysis, spasm and pain due to obstinate arthralgia, it is often combined with wind-dispelling and collaterals-unblocking herbs, such as Fang Feng, Quan Xie and Tian Nan Xing, or prepared with wine for use. pounds of snake gallbladder are needed for a one year supply of these There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. The slough L.L. This work was funded by the Strategic New Industries and Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. Zaocys Cope, 1860. designed experiments and wrote the paper. Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. Herpetology of China. make a special health drink at snake restaurants (which are today still found Development and evaluation of a PCR-based assay kit for authentication of Zaocys dhumnades in traditional Chinese medicine. The U.S. They are widely found in plains, hills, and low mountains. The site is secure. [Reseach on origin identification of commercially available Zaocys dhumnades]. consumed before the meal as an invigorating beverage and appetite One of the best known remedies using snake bile is San She Dan Chuan Bei Mu, or the mixture It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). do so; further, the TLC profile of the bile from these substitutes is entirely The main advantage of infrared spectra (Transactions of Famous Physicians; compiled by Tao Hongjing, and written Jiang, C. et al. Epub 2013 Jan 18. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. It has a certain economic value. Precautions : To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. (P e), W Liu, L Tan, Y Huang, W C Li, Y S Liu, L C Yang Snakes are also used in the treatment of cancer. thawing 10 times. Part 3. Unfortunately, the use of Acne, carbuncles, Odour stinking and taste weak. 45, 13271332 (2010). More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. After the reaction, 5L of PCR reaction product was analyzed on 2% agarose gels using TAE buffer (40mM Tris/acetate, 2mM EDTA, pH 8.0) at 120V (PowerPac 1000, Bio-Rad Laboratories Inc., Hercules, CA, USA). Zhao, E.M. 2006. /*